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PAK1/PAK2/PAK3 (phospho S144/S141/S139) monoclonal antibody, clone CFF-16 

  • Catalog # : MAB20548
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic phosphopeptide of human PAK1/PAK2/PAK3.
  • Immunogen:
  • A synthetic phosphopeptide corresponding to residues surrounding S144/S141/S139 of human PAK1/PAK2/PAK3.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Flow Cytometry (1:50)
    Immunocytochemistry (1:50-1:200)
    Immunofluorescence (1:50-1:200)
    Immunohistochemistry (1:50-1:200)
    Immunoprecipitation (1:50)
    Western Blot (1:1000-1:10000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.4-0.5 mg/mL BSA, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of HeLa Cell lysate treated with lambda phosphatase using PAK1/PAK2/PAK3 (phospho S144/S141/S139) monoclonal antibody, clone CFF-16.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using PAK1/PAK2/PAK3 (phospho S144/S141/S139) monoclonal antibody, clone CFF-16.
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 5058
  • Gene Name:
  • PAK1
  • Gene Alias:
  • MGC130000,MGC130001,PAKalpha
  • Gene Description:
  • p21 protein (Cdc42/Rac)-activated kinase 1
  • Gene Summary:
  • PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • STE20 homolog, yeast,p21-activated kinase 1,p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast),p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
  • Gene Information
  • Entrez GeneID:
  • 5062
  • Gene Name:
  • PAK2
  • Gene Alias:
  • PAK65,PAKgamma
  • Gene Description:
  • p21 protein (Cdc42/Rac)-activated kinase 2
  • Gene Summary:
  • The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq
  • Other Designations:
  • S6/H4 kinase,p21 (CDKN1A)-activated kinase 2,p21-activated kinase 2
  • Gene Information
  • Entrez GeneID:
  • 5063
  • Gene Name:
  • PAK3
  • Gene Alias:
  • CDKN1A,MRX30,MRX47,OPHN3,PAK3beta,bPAK,hPAK3
  • Gene Description:
  • p21 protein (Cdc42/Rac)-activated kinase 3
  • Gene Summary:
  • PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The protein encoded by this gene forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of non-syndromic mental retardation X-linked type 30 (MRX30), also called X-linked mental retardation type 47 (MRX47). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000023855,OTTHUMP00000062894,beta-PAK,oligophrenin-3,p21 (CDKN1A)-activated kinase 3,p21-activated kinase 3,p21-activated kinase-3,serine/threonine-protein kinase PAK 3
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