PAK1/PAK2/PAK3 (phospho S144/S141/S139) monoclonal antibody, clone CFF-16
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of HeLa Cell lysate treated with lambda phosphatase using PAK1/PAK2/PAK3 (phospho S144/S141/S139) monoclonal antibody, clone CFF-16.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded mouse brain using PAK1/PAK2/PAK3 (phospho S144/S141/S139) monoclonal antibody, clone CFF-16.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human PAK1/PAK2/PAK3.
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding S144/S141/S139 of human PAK1/PAK2/PAK3.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50)
Western Blot (1:1000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.4-0.5 mg/mL BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of HeLa Cell lysate treated with lambda phosphatase using PAK1/PAK2/PAK3 (phospho S144/S141/S139) monoclonal antibody, clone CFF-16.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded mouse brain using PAK1/PAK2/PAK3 (phospho S144/S141/S139) monoclonal antibody, clone CFF-16.Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — PAK1
Entrez GeneID
5058Protein Accession#
O75914Gene Name
PAK1
Gene Alias
MGC130000, MGC130001, PAKalpha
Gene Description
p21 protein (Cdc42/Rac)-activated kinase 1
Omim ID
602590Gene Ontology
HyperlinkGene Summary
PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
STE20 homolog, yeast|p21-activated kinase 1|p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)|p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
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Gene Info — PAK2
Entrez GeneID
5062Protein Accession#
O75914Gene Name
PAK2
Gene Alias
PAK65, PAKgamma
Gene Description
p21 protein (Cdc42/Rac)-activated kinase 2
Omim ID
605022Gene Ontology
HyperlinkGene Summary
The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq
Other Designations
S6/H4 kinase|p21 (CDKN1A)-activated kinase 2|p21-activated kinase 2
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Gene Info — PAK3
Entrez GeneID
5063Protein Accession#
O75914Gene Name
PAK3
Gene Alias
CDKN1A, MRX30, MRX47, OPHN3, PAK3beta, bPAK, hPAK3
Gene Description
p21 protein (Cdc42/Rac)-activated kinase 3
Gene Ontology
HyperlinkGene Summary
PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The protein encoded by this gene forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of non-syndromic mental retardation X-linked type 30 (MRX30), also called X-linked mental retardation type 47 (MRX47). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000023855|OTTHUMP00000062894|beta-PAK|oligophrenin-3|p21 (CDKN1A)-activated kinase 3|p21-activated kinase 3|p21-activated kinase-3|serine/threonine-protein kinase PAK 3
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Interactome
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Pathway
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Disease
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