PAK1 recombinant monoclonal antibody, clone 4F10
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: NIH/3T3 whole cell lysate; Lane 2: MCF-7 whole cell lysate; Lane 3: ntera-2 whole cell lysate; Lane 4: Mouse brain tissue.
Immunofluorescence
Immunofluorescence staining of Hela Cells with PAK1 recombinant monoclonal antibody, clone 4F10 at 1:50, counter-stained with DAPI.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human and mouse PAK1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human PAK1.
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence(1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: NIH/3T3 whole cell lysate; Lane 2: MCF-7 whole cell lysate; Lane 3: ntera-2 whole cell lysate; Lane 4: Mouse brain tissue.Immunofluorescence
Immunofluorescence staining of Hela Cells with PAK1 recombinant monoclonal antibody, clone 4F10 at 1:50, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PAK1
Entrez GeneID
5058Protein Accession#
Q13153Gene Name
PAK1
Gene Alias
MGC130000, MGC130001, PAKalpha
Gene Description
p21 protein (Cdc42/Rac)-activated kinase 1
Omim ID
602590Gene Ontology
HyperlinkGene Summary
PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
STE20 homolog, yeast|p21-activated kinase 1|p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)|p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
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Interactome
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Pathway
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Disease
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