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Last updated: 2021/8/8
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Hemoglobin Alpha Chain monoclonal antibody, clone EGI-8 

  • Catalog # : MAB20135
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human Hemoglobin Alpha Chain .
  • Immunogen:
  • A synthetic peptide corresponding to human Hemoglobin Alpha Chain .
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 15.258
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Flow Cytometry (1:50)
    Immunohistochemistry (1:50-1:200)
    Immunoprecipitation (1:50)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of K562 cell lysate with Hemoglobin Alpha Chain monoclonal antibody, clone EGI-8 (Cat # MAB20135).
  • Immunohistochemistry
  • Immunoprecipitation
  • Flow Cytometry
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • Immunoprecipitation
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 3039
  • Gene Name:
  • HBA1
  • Gene Alias:
  • HBH,HBA-T3
  • Gene Description:
  • hemoglobin, alpha 1
  • Gene Summary:
  • The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported. [provided by RefSeq
  • Other Designations:
  • alpha 1 globin,alpha one globin,alpha-1 globin,alpha-1-globin,hemoglobin alpha 1 globin chain,hemoglobin alpha-1 chain
  • Gene Information
  • Entrez GeneID:
  • 3040
  • Gene Name:
  • HBA2
  • Gene Alias:
  • -
  • Gene Description:
  • hemoglobin, alpha 2
  • Gene Summary:
  • The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported. [provided by RefSeq
  • Other Designations:
  • alpha 2 globin,alpha globin,alpha-2 globin
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