Hemoglobin Alpha Chain monoclonal antibody, clone EGI-8
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of K562 cell lysate with Hemoglobin Alpha Chain monoclonal antibody, clone EGI-8 (Cat # MAB20135).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human Hemoglobin Alpha Chain.
Immunogen
A synthetic peptide corresponding to human Hemoglobin Alpha Chain .
Host
Rabbit
Theoretical MW (kDa)
15.258
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of K562 cell lysate with Hemoglobin Alpha Chain monoclonal antibody, clone EGI-8 (Cat # MAB20135).Immunohistochemistry
Immunoprecipitation
Flow Cytometry
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Gene Info — HBA1
Entrez GeneID
3039Protein Accession#
P69905Gene Name
HBA1
Gene Alias
HBH, HBA-T3
Gene Description
hemoglobin, alpha 1
Omim ID
141800Gene Ontology
HyperlinkGene Summary
The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported. [provided by RefSeq
Other Designations
alpha 1 globin|alpha one globin|alpha-1 globin|alpha-1-globin|hemoglobin alpha 1 globin chain|hemoglobin alpha-1 chain
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Gene Info — HBA2
Entrez GeneID
3040Protein Accession#
P69905Gene Name
HBA2
Gene Alias
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Gene Description
hemoglobin, alpha 2
Omim ID
141850Gene Ontology
HyperlinkGene Summary
The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported. [provided by RefSeq
Other Designations
alpha 2 globin|alpha globin|alpha-2 globin
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Interactome
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Disease
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