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Last updated: 2021/8/8
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CTCF monoclonal antibody, clone AAAE-3 

  • Catalog # : MAB19923
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human CTCF.
  • Immunogen:
  • A synthetic peptide corresponding to human CTCF.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Flow Cytometry (1:50)
    Immunocytochemistry (1:50-1:200)
    Immunofluorescence (1:50-1:200)
    Immunohistochemistry (1:100-1:500)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Instruction:
  • Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of (1) HeLa cell lysate; (2) Mouse brain lysate with CTCF monoclonal antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Immunofluorescence
  • Flow Cytometry
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Immunofluorescence
  • Flow Cytometry
  • Gene Information
  • Gene Name:
  • CTCF
  • Gene Alias:
  • -
  • Gene Description:
  • CCCTC-binding factor (zinc finger protein)
  • Gene Summary:
  • This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. [provided by RefSeq
  • Other Designations:
  • 11 zinc finger transcriptional repressor,11-zinc finger protein,CCCTC-binding factor,CTCFL paralog,transcriptional repressor CTCF
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