Western blot analysis of human MSLN recombinant protein at 100 ng (lane 1), 25 ng (lane 2), 6 ng (lane 3), 2 ng (lane 4) and 0.4 ng (lane 5) with MSLN monoclonal antibodies at 1:1000 dilution. Lane 6 contains 50 ng of human CDC25 recombinant protein. Top panel: Cat # MAB1993. Bottom panel: Cat # MAB1992.
An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Alternative splicing results in multiple transcript variants. [provided by RefSeq