MSLN monoclonal antibody, clone MB-G10
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis of human MSLN recombinant protein at 100 ng (lane 1), 25 ng (lane 2), 6 ng (lane 3), 2 ng (lane 4) and 0.4 ng (lane 5) with MSLN monoclonal antibodies at 1:1000 dilution. Lane 6 contains 50 ng of human CDC25 recombinant protein.
Top panel: Cat # MAB1993.
Bottom panel: Cat # MAB1992.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human mesothelioma tissues with MSLN monoclonal antibodies at 1:100 dilution.
Top panel: Cat # MAB1992.
Bottom panel: Cat # MAB1993.
-
Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant MSLN.
Immunogen
Recombinant protein corresponding to extracellular region of human MSLN.
Host
Mouse
Reactivity
Human
Specificity
This antibody is directed against human mesothelin protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Recombinant protein)
Western blot analysis of human MSLN recombinant protein at 100 ng (lane 1), 25 ng (lane 2), 6 ng (lane 3), 2 ng (lane 4) and 0.4 ng (lane 5) with MSLN monoclonal antibodies at 1:1000 dilution. Lane 6 contains 50 ng of human CDC25 recombinant protein.
Top panel: Cat # MAB1993.
Bottom panel: Cat # MAB1992.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human mesothelioma tissues with MSLN monoclonal antibodies at 1:100 dilution.
Top panel: Cat # MAB1992.
Bottom panel: Cat # MAB1993.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — MSLN
Entrez GeneID
10232Protein Accession#
NP_005814;Q13421Gene Name
MSLN
Gene Alias
CAK1, MPF, SMR
Gene Description
mesothelin
Omim ID
601051Gene Ontology
HyperlinkGene Summary
An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
megakaryocyte potentiating factor
-
Interactome
-
Disease
-
Publication Reference
-
Mesothelin expression in human lung cancer.
Ho M, Bera TK, Willingham MC, Onda M, Hassan R, FitzGerald D, Pastan I.
Clinical Cancer Research 2007 Mar; 13(5):1571.
Application：Flow, Cyt, IHC-P, WB, Human , A-549, EKVX, NCI-H322M, NCI-H522, NCI-H460 cells, Human lung cancer tissues.
-
New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA.
Onda M, Willingham M, Nagata S, Bera TK, Beers R, Ho M, Hassan R, Kreitman RJ, Pastan I.
Clinical Cancer Research 2005 Aug; 11(16):5840.
Application：ELISA, Flow Cyt, IHC-P, WB-Ce, Human, A1847, H226, HeLa, Panc3.014 cells, Human ovarian cancer.
-
Isolation and characterization of a monoclonal antibody, K1, reactive with ovarian cancers and normal mesothelium.
Chang K, Pastan I, Willingham MC.
International Journal of Cancer 1992 Feb; 50(3):373.
-
Mesothelin expression in human lung cancer.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com