MSLN recombinant monoclonal antibody, clone 11-25
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More Files
- More Functions
Immunofluorescence
Immunofluorescent staining of Hela cells with MSLN recombinant monoclonal antibody, clone 11-25 (Cat # RAB03642)
Immunofluorescence analysis of paraformaldehyde fixed Hela cells stained with the chimeric r version of RAB03642 at a dilution of 1:100 for 1h followed by Alexa Fluor® 488 secondary antibody at a dilution of 1:1000, showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.
(A) RAB03642
(B) DAPI
(C) Merged channels
(D) Isotype control
Flow Cytometry
Flow cytometric analysis of Hela cells with MSLN recombinant monoclonal antibody, clone 11-25 (Cat # RAB03642).
Paraformaldehyde fixed Hela cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control-black line) or the r version of RAB03642 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human MSLN.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against the purified recombinant protein corresponding to human MSLN.
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunofluorescence
Immunoprecipitation
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescent staining of Hela cells with MSLN recombinant monoclonal antibody, clone 11-25 (Cat # RAB03642)
Immunofluorescence analysis of paraformaldehyde fixed Hela cells stained with the chimeric r version of RAB03642 at a dilution of 1:100 for 1h followed by Alexa Fluor® 488 secondary antibody at a dilution of 1:1000, showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.
(A) RAB03642
(B) DAPI
(C) Merged channels
(D) Isotype controlImmunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Hela cells with MSLN recombinant monoclonal antibody, clone 11-25 (Cat # RAB03642).
Paraformaldehyde fixed Hela cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control-black line) or the r version of RAB03642 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — MSLN
Entrez GeneID
10232Gene Name
MSLN
Gene Alias
CAK1, MPF, SMR
Gene Description
mesothelin
Omim ID
601051Gene Ontology
HyperlinkGene Summary
An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
megakaryocyte potentiating factor
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Interactome
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Disease
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