CDC40 monoclonal antibody, clone IAO-3
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More Files
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human CDC40.
Immunogen
A synthetic peptide corresponding to human CDC40.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) HeLa cell lysate; (2) K562 cell lysate with CDC40 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — CDC40
Entrez GeneID
51362Protein Accession#
O60508Gene Name
CDC40
Gene Alias
EHB3, FLJ10564, MGC102802, PRP17, PRPF17
Gene Description
cell division cycle 40 homolog (S. cerevisiae)
Omim ID
605585Gene Ontology
HyperlinkGene Summary
Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp17 protein, which functions in two different cellular processes: pre-mRNA splicing and cell cycle progression. It suggests that this protein may play a role in cell cycle progression. [provided by RefSeq
Other Designations
EH-binding protein 3|OTTHUMP00000016997|cell division cycle 40 homolog|pre-mRNA splicing factor 17
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Interactome
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Disease
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