CDC34 monoclonal antibody, clone IBO-3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate with CDC34 monoclonal antibody.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human CDC34.
Immunogen
A synthetic peptide corresponding to human CDC34.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunoprecipitation (1:50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate with CDC34 monoclonal antibody.Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — CDC34
Entrez GeneID
997Protein Accession#
P49427Gene Name
CDC34
Gene Alias
E2-CDC34, UBC3, UBE2R1
Gene Description
cell division cycle 34 homolog (S. cerevisiae)
Omim ID
116948Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq
Other Designations
ubiquitin carrier protein|ubiquitin-conjugating enzyme Cdc34|ubiquitin-conjugating enzyme E2-32 KDA complementing|ubiquitin-protein ligase
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Interactome
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Pathway
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Disease
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