CD34 polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Human cell line RT-4 with CD34 polyclonal antibody (Cat# PAB29333) at 1:100-1:250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney with CD34 polyclonal antibody (Cat# PAB29333) shows strong cytoplasmic positivity in cells of tubules at 1:50-1:200 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with CD34 polyclonal antibody (Cat# PAB29333) under 1-4 ug/mL working concentration shows positivity in cytoplasm and nucleus but excluded from the nucleoli.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human CD34.
Immunogen
Recombinant protein corresponding to amino acids of human CD34.
Sequence
LNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGT
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:100-1:250)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Human cell line RT-4 with CD34 polyclonal antibody (Cat# PAB29333) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney with CD34 polyclonal antibody (Cat# PAB29333) shows strong cytoplasmic positivity in cells of tubules at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with CD34 polyclonal antibody (Cat# PAB29333) under 1-4 ug/mL working concentration shows positivity in cytoplasm and nucleus but excluded from the nucleoli. -
Gene Info — CDC34
Entrez GeneID
997Protein Accession#
P49427Gene Name
CDC34
Gene Alias
E2-CDC34, UBC3, UBE2R1
Gene Description
cell division cycle 34 homolog (S. cerevisiae)
Omim ID
116948Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq
Other Designations
ubiquitin carrier protein|ubiquitin-conjugating enzyme Cdc34|ubiquitin-conjugating enzyme E2-32 KDA complementing|ubiquitin-protein ligase
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Interactome
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Pathway
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Disease
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