CDC25C monoclonal antibody, clone HIG-3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of K562 cell lysate with CDC25C monoclonal antibody.
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Specifications
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human CDC25C.
Immunogen
A synthetic peptide corresponding to human CDC25C.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:10-1:500)
Immunoprecipitation (1:30)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of K562 cell lysate with CDC25C monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — CDC25C
Entrez GeneID
995Protein Accession#
P30307Gene Name
CDC25C
Gene Alias
CDC25
Gene Description
cell division cycle 25 homolog C (S. pombe)
Omim ID
157680Gene Ontology
HyperlinkGene Summary
This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq
Other Designations
cell division cycle 25C|cell division cycle 25C protein|dual specificity phosphatase CDC25C|m-phase inducer phosphatase 3|mitosis inducer CDC25|phosphotyrosine phosphatase
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Interactomes
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Pathways
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Diseases
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