CDC25C recombinant monoclonal antibody, clone 3E6
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: K562 whole cell lysate, Lane 3: Raji whole cell lysate and Lane 4: 293 whole cell lysate with CDC25C recombinant monoclonal antibody, clone 3E6 (Cat # RAB04342).
Immunohistochemistry
Immunohistochemical staining of human glioma cancer with CDC25C recombinant monoclonal antibody, clone 3E6 (Cat # RAB04342) (diluated at 1:165).
Immunofluorescence
Immunofluorescent staining of Hela cells with CDC25C recombinant monoclonal antibody, clone 3E6 (Cat # RAB04342) (diluated at 1:55). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Immunoprecipitation
Immunoprecipitation analysis of HEK293 cell lysate with CDC25C recombinant monoclonal antibody, clone 3E6 (Cat # RAB04342).
Lane 1: rabbit control IgG, Lane 2: RAB04342 precipitates and Lane 3: Input (HEK293 whole cell lysate).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CDC25C.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CDC25C.
Theoretical MW (kDa)
Calculated MW: 54, 5
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: K562 whole cell lysate, Lane 3: Raji whole cell lysate and Lane 4: 293 whole cell lysate with CDC25C recombinant monoclonal antibody, clone 3E6 (Cat # RAB04342).Immunohistochemistry
Immunohistochemical staining of human glioma cancer with CDC25C recombinant monoclonal antibody, clone 3E6 (Cat # RAB04342) (diluated at 1:165).Immunofluorescence
Immunofluorescent staining of Hela cells with CDC25C recombinant monoclonal antibody, clone 3E6 (Cat # RAB04342) (diluated at 1:55). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Immunoprecipitation
Immunoprecipitation analysis of HEK293 cell lysate with CDC25C recombinant monoclonal antibody, clone 3E6 (Cat # RAB04342).
Lane 1: rabbit control IgG, Lane 2: RAB04342 precipitates and Lane 3: Input (HEK293 whole cell lysate).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDC25C
Entrez GeneID
995Protein Accession#
P30307Gene Name
CDC25C
Gene Alias
CDC25
Gene Description
cell division cycle 25 homolog C (S. pombe)
Omim ID
157680Gene Ontology
HyperlinkGene Summary
This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq
Other Designations
cell division cycle 25C|cell division cycle 25C protein|dual specificity phosphatase CDC25C|m-phase inducer phosphatase 3|mitosis inducer CDC25|phosphotyrosine phosphatase
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Interactome
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Pathway
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Disease
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