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Last updated: 2020/11/29

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SMARCA4 monoclonal antibody, clone GFB-19 

  • Catalog # : MAB19675
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human SMARCA4.
  • Immunogen:
  • A synthetic peptide corresponding to human SMARCA4.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 184.646
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ChIP
    Immunocytochemistry (1:50-1:200)
    Immunofluorescence (1:50-1:200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
    Immunoprecipitation (1:50)
    Western Blot (1:5000-1:20000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ChIP
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of HeLa cell lysate with SMARCA4 monoclonal antibody, clone GFB-19 (Cat # MAB19675).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Application Image
  • ChIP
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 6597
  • Gene Name:
  • SMARCA4
  • Gene Alias:
  • BAF190,BRG1,FLJ39786,SNF2,SNF2-BETA,SNF2L4,SNF2LB,SWI2,hSNF2b
  • Gene Description:
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
  • Gene Summary:
  • The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • ATP-dependent helicase SMARCA4,BRM/SWI2-related gene 1,SNF2-like 4,SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a4,brahma protein-like 1,global transcription activator homologous sequence,homeotic gene regulator,mitotic growth
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