SMARCA4 recombinant monoclonal antibody, clone 22F3
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More Files
- More Functions
Immunohistochemistry
Immunohistochemistry image of SMARCA4 recombinant monoclonal antibody, clone 22F3 diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system.
Immunofluorescence
Immunofluorescence staining of Hela Cells with SMARCA4 recombinant monoclonal antibody, clone 22F3 at 1:20, counter-stained with DAPI.
Flow Cytometry
Overlay Peak curve showing Hela cells stained with SMARCA4 recombinant monoclonal antibody, clone 22F3 (red line) at 1:50.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human SMARCA4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human SMARCA4.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry(1:50-1:200)
Immunohistochemistry(1:50-1:200)
Immunofluorescence(1:20-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry
Immunohistochemistry image of SMARCA4 recombinant monoclonal antibody, clone 22F3 diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system.Immunofluorescence
Immunofluorescence staining of Hela Cells with SMARCA4 recombinant monoclonal antibody, clone 22F3 at 1:20, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay Peak curve showing Hela cells stained with SMARCA4 recombinant monoclonal antibody, clone 22F3 (red line) at 1:50. -
Gene Info — SMARCA4
Entrez GeneID
6597Protein Accession#
P51532Gene Name
SMARCA4
Gene Alias
BAF190, BRG1, FLJ39786, SNF2, SNF2-BETA, SNF2L4, SNF2LB, SWI2, hSNF2b
Gene Description
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Omim ID
603254Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
ATP-dependent helicase SMARCA4|BRM/SWI2-related gene 1|SNF2-like 4|SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a4|brahma protein-like 1|global transcription activator homologous sequence|homeotic gene regulator|mitotic growth
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Interactome
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Disease
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