ENO2 monoclonal antibody, clone NSE-P2
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis using ENO2 monoclonal antibody, clone NSE-P2 (Cat # MAB1963) on gamma-enolase, 100 ng (lane 1) and alpha-enolase, 500 ng (lane 2) standards.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of normal pancreas tissue (A) and small bowel tumor tissue (B) using ENO2 monoclonal antibody, clone NSE-P2 (Cat # MAB1963).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of ENO2.
Immunogen
A synthetic peptide corresponding to human ENO2.
Host
Mouse
Reactivity
Chicken, Human, Mouse
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western blot (1 to 2 ug/mL)
Immunohistochemistry on formalin to fixed/paraffin to embedded tissues (1 to 5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.08% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis using ENO2 monoclonal antibody, clone NSE-P2 (Cat # MAB1963) on gamma-enolase, 100 ng (lane 1) and alpha-enolase, 500 ng (lane 2) standards.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of normal pancreas tissue (A) and small bowel tumor tissue (B) using ENO2 monoclonal antibody, clone NSE-P2 (Cat # MAB1963).Enzyme-linked Immunoabsorbent Assay
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Gene Info — ENO2
Entrez GeneID
2026Gene Name
ENO2
Gene Alias
NSE
Gene Description
enolase 2 (gamma, neuronal)
Omim ID
131360Gene Ontology
HyperlinkGene Summary
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq
Other Designations
2-phospho-D-glycerate hydrolyase|enolase 2|neural enolase|neuron specific gamma enolase|neurone-specific enolase
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Glycolysis / Gluconeogenesis
- Metabolic pathways
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Disease
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Publication Reference
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Plasma S100beta and NSE levels and progression in multiple sclerosis.
Koch M, Mostert J, Heersema D, Teelken A, De Keyser J.
Journal of the Neurological Sciences 2007 Jan; 252(2):154.
Application：ELISA, Human, Plasma from patients with multiple sclerosis.
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S-100B and NSE: markers of initial impact of subarachnoid haemorrhage and their relation to vasospasm and outcome.
Oertel M, Schumacher U, McArthur DL, Kästner S, Böker DK.
Journal of Clinical Neuroscience 2006 Oct; 13(8):834.
Application：RIA, Human, Serum from patients with subarachnoid haemorrhage.
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Immunoreactive neuron-specific enolase (NSE) is expressed in testicular carcinoma-in-situ.
Kang JL, Meyts ER, Skakkebaek NE.
The Journal of Pathology 1996 Feb; 178(2):161.
Application：IHC-P, Human, Overt germ cell tumours, Testicular carcinoma, Testes.
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Plasma S100beta and NSE levels and progression in multiple sclerosis.
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