ATPIF1 monoclonal antibody, clone 1F3B8
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human ATPIF1.
Immunogen
Recombinant protein corresponding to full length human ATPIF1.
Host
Mouse
Theoretical MW (kDa)
12.3
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunofluorescence (1:100-1:500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: ATPIF1-hIgGFc transfected HEK293 cell lysates with ATPIF1 monoclonal antibody, clone 1F3B8 (Cat # MAB17937).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung cancer with ATPIF1 monoclonal antibody, clone 1F3B8 (Cat # MAB17937).Immunofluorescence
Immunofluorescent staining of HeLa cells with ATPIF1 monoclonal antibody, clone 1F3B8 (Cat # MAB17937) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis with ATPIF1 monoclonal antibody, clone 1F3B8 (Cat # MAB17937).Flow Cytometry
Flow cytometric analysis of HeLa cells with ATPIF1 monoclonal antibody, clone 1F3B8 (Cat # MAB17937) (Green). Red: Negative Control. -
Gene Info — ATPIF1
Entrez GeneID
93974Gene Name
ATPIF1
Gene Alias
ATPI, ATPIP, IP, MGC1167, MGC8898
Gene Description
ATPase inhibitory factor 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. [provided by RefSeq
Other Designations
ATP synthase inhibitor protein|ATPase inhibitor protein|OTTHUMP00000003642
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Interactome
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Disease
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