AXIN1 monoclonal antibody, clone 4E9F1
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: AXIN1-hIgGFc transfected HEK293 cell lysates with AXIN1 monoclonal antibody, clone 4E9F1 (Cat # MAB17915).
Immunofluorescence
Immunofluorescent staining of HeLa cells with AXIN1 monoclonal antibody, clone 4E9F1 (Cat # MAB17915) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with AXIN1 monoclonal antibody, clone 4E9F1 (Cat # MAB17915).
Flow Cytometry
Flow cytometric analysis of HeLa cells with AXIN1 monoclonal antibody, clone 4E9F1 (Cat # MAB17915) (Green). Red: Negative Control.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human AXIN1.
Immunogen
Recombinant protein corresponding to amino acids 546-752 of human AXIN1.
Host
Mouse
Theoretical MW (kDa)
95.6
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunofluorescence (1:50-1:250)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: AXIN1-hIgGFc transfected HEK293 cell lysates with AXIN1 monoclonal antibody, clone 4E9F1 (Cat # MAB17915).Immunofluorescence
Immunofluorescent staining of HeLa cells with AXIN1 monoclonal antibody, clone 4E9F1 (Cat # MAB17915) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis with AXIN1 monoclonal antibody, clone 4E9F1 (Cat # MAB17915).Flow Cytometry
Flow cytometric analysis of HeLa cells with AXIN1 monoclonal antibody, clone 4E9F1 (Cat # MAB17915) (Green). Red: Negative Control. -
Gene Info — AXIN1
Entrez GeneID
8312Protein Accession#
O15169Gene Name
AXIN1
Gene Alias
AXIN, MGC52315
Gene Description
axin 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin (cadherin-associated protein), beta 1, 88kDa, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
axis inhibition protein 1|axis inhibitor 1|fused, mouse, homolog of
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Interactome
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Pathway
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Disease
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