IDH1 monoclonal antibody, clone 7G8A1
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot (Transfected lysate) analysis of (1) HEK293, (2) IDH1-hIgGFc transfected HEK293 cell lysate.
Western Blot
Western Blot analysis of (1) HepG2, (2) NIH/3T3, (3) C2C12, (4) COS7, (5) SW480 cell lysate.
Immunofluorescence
Immunofluorescent staining of HeLa cell line with antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of IDH1 monoclonal antibody.
Flow Cytometry
Flow cytometric analysis of HeLa cells using IDH1 mouse monoclonal antibody (green) and negative control (red).
-
Specification
Product Description
Mouse monoclonal antibody raised against recombinant human IDH1.
Immunogen
Recombinant protein corresponding to amino acids 156-298 of human IDH1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
46.7
Reactivity
Human, Monkey, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-400)
Immunofluorescence (1:50-250)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Transfected lysate)
Western Blot (Transfected lysate) analysis of (1) HEK293, (2) IDH1-hIgGFc transfected HEK293 cell lysate.Western Blot
Western Blot analysis of (1) HepG2, (2) NIH/3T3, (3) C2C12, (4) COS7, (5) SW480 cell lysate.Immunofluorescence
Immunofluorescent staining of HeLa cell line with antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of IDH1 monoclonal antibody.Flow Cytometry
Flow cytometric analysis of HeLa cells using IDH1 mouse monoclonal antibody (green) and negative control (red). -
Gene Info — IDH1
Entrez GeneID
3417Gene Name
IDH1
Gene Alias
IDCD, IDH, IDP, IDPC, PICD
Gene Description
isocitrate dehydrogenase 1 (NADP+), soluble
Omim ID
147700Gene Ontology
HyperlinkGene Summary
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq
Other Designations
NADP+-specific ICDH|NADP-dependent isocitrate dehydrogenase, cytosolic|NADP-dependent isocitrate dehydrogenase, peroxisomal|oxalosuccinate decarboxylase
-
Interactome
-
Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Glutathione metabolism
+ View More Disease
-
Disease
- Adenoma
- Astrocytoma
- Blast Crisis
- Brain Neoplasms
- Carcinoma
- Chronic Disease
- Cleft Lip
- Cleft Palate
- Disease Progression
+ View More Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com