IDH1 recombinant monoclonal antibody, clone R05-2G5
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More Files
- More Functions
Western Blot
Western blot analysis of IDH1 in rat Brain, C6, 3T3, Hela lysates using human Isocitrate dehydrogenase recombinant monoclonal antibody, clone R05-2G5 (Cat # RAB01738).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of Human Cholangiocarcinoma with Isocitrate dehydrogenase recombinant monoclonal antibody, clone R05-2G5 (Cat # RAB01738).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human Isocitrate dehydrogenase.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human Isocitrate dehydrogenase
Theoretical MW (kDa)
Calculated MW: 47 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1,000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of IDH1 in rat Brain, C6, 3T3, Hela lysates using human Isocitrate dehydrogenase recombinant monoclonal antibody, clone R05-2G5 (Cat # RAB01738).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of Human Cholangiocarcinoma with Isocitrate dehydrogenase recombinant monoclonal antibody, clone R05-2G5 (Cat # RAB01738).Immunoprecipitation
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Gene Info — IDH1
Entrez GeneID
3417Protein Accession#
O75874Gene Name
IDH1
Gene Alias
IDCD, IDH, IDP, IDPC, PICD
Gene Description
isocitrate dehydrogenase 1 (NADP+), soluble
Omim ID
147700Gene Ontology
HyperlinkGene Summary
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq
Other Designations
NADP+-specific ICDH|NADP-dependent isocitrate dehydrogenase, cytosolic|NADP-dependent isocitrate dehydrogenase, peroxisomal|oxalosuccinate decarboxylase
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Glutathione metabolism
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Disease
- Adenoma
- Astrocytoma
- Blast Crisis
- Brain Neoplasms
- Carcinoma
- Chronic Disease
- Cleft Lip
- Cleft Palate
- Disease Progression
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