NEFM monoclonal antibody, clone 1B6B12
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) NEFM-hIgGFc transfected HEK293 cell lysate with NEFM monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with NEFM monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of NEFM monoclonal antibody, clone 1B6B12.
Flow Cytometry
Flow cytometric analysis of HeLa cells with NEFM monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human NEFM.
Immunogen
Recombinant protein corresponding to amino acids 779-916 of human NEFM from E. coli.
Host
Mouse
Theoretical MW (kDa)
110
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry (1:50-1:200)
Immunohistochemistry
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) NEFM-hIgGFc transfected HEK293 cell lysate with NEFM monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with NEFM monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of NEFM monoclonal antibody, clone 1B6B12.Flow Cytometry
Flow cytometric analysis of HeLa cells with NEFM monoclonal antibody (green) and negative control (red). -
Gene Info — NEFM
Entrez GeneID
4741Gene Name
NEFM
Gene Alias
NEF3, NF-M, NFM
Gene Description
neurofilament, medium polypeptide
Omim ID
162250Gene Ontology
HyperlinkGene Summary
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
neurofilament triplet M protein|neurofilament, medium polypeptide 150kDa|neurofilament-3 (150 kD medium)
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Interactome
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Pathway
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Disease
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