E2F1 monoclonal antibody, clone 8G9
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) HeLa cell, (2) SK-N-SH cell, (3) NIH/3T3 cell with E2F1 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of E2F1 monoclonal antibody, clone 8G9.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human E2F1.
Immunogen
Recombinant protein corresponding to amino acids 69-223 of human E2F1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
46.9
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) HeLa cell, (2) SK-N-SH cell, (3) NIH/3T3 cell with E2F1 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of E2F1 monoclonal antibody, clone 8G9. -
Gene Info — E2F1
Entrez GeneID
1869Gene Name
E2F1
Gene Alias
E2F-1, RBAP1, RBBP3, RBP3
Gene Description
E2F transcription factor 1
Omim ID
189971Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq
Other Designations
OTTHUMP00000030661|retinoblastoma-associated protein 1
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Interactome
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Pathway
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Disease
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