CHRNA5 monoclonal antibody, clone 8F11G8
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) membrane protein lysate of C6, (2) membrane protein lysate of SK-N-SH, (3) membrane protein lysate of C6, (4) C6 cell with CHRNA5 monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) CHRNA5-hIgGFc transfected HEK293 cell lysate.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CHRNA5 monoclonal antibody, clone 8F11G8.
Flow Cytometry
Flow cytometric analysis of SK-N-SH cells with CHRNA5 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human CHRNA5.
Immunogen
Recombinant protein corresponding to amino acid 23-254 of human CHRNA5 from E. coli.
Host
Mouse
Theoretical MW (kDa)
53
Reactivity
Human, Rat
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry
Flow Cytometry (1:200-1:400)
Immunohistochemistry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) membrane protein lysate of C6, (2) membrane protein lysate of SK-N-SH, (3) membrane protein lysate of C6, (4) C6 cell with CHRNA5 monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) CHRNA5-hIgGFc transfected HEK293 cell lysate.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CHRNA5 monoclonal antibody, clone 8F11G8.Flow Cytometry
Flow cytometric analysis of SK-N-SH cells with CHRNA5 monoclonal antibody (green) and negative control (red). -
Gene Info — CHRNA5
Entrez GeneID
1138Gene Name
CHRNA5
Gene Alias
LNCR2
Gene Description
cholinergic receptor, nicotinic, alpha 5
Omim ID
118505Gene Ontology
HyperlinkGene Summary
Nicotinic acetylcholine receptors (nAChRs), such as CHRNA5, are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be (hetero)pentamers composed of homologous subunits. See MIM 118508 for additional background information on AChRs.[supplied by OMIM
Other Designations
Cholinergic receptor, neuronal nicotinic, alpha polypeptide-5|cholinergic receptor, nicotinic, alpha polypeptide 5|neuronal nicotinic acetylcholine receptor, alpha5 subunit
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Interactome
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Disease
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