Toxoplasma gondii ROP1 monoclonal antibody, clone 3D4B2
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) ROP1-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissue with Toxoplasma gondii ROP1 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of SMMC-7721 cells with Toxoplasma gondii ROP1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Toxoplasma gondii ROP1 monoclonal antibody, clone 3D4B2.
-
Specification
Product Description
Mouse monoclonal antibody raised against Toxoplasma gondii rhoptry protein ROP1.
Immunogen
Recombinant protein corresponding to amino acid 42-183 of human Toxoplasma gondii rhoptry protein ROP1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
42.6
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) ROP1-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissue with Toxoplasma gondii ROP1 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of SMMC-7721 cells with Toxoplasma gondii ROP1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Toxoplasma gondii ROP1 monoclonal antibody, clone 3D4B2. -
Gene Info — ROP1
-
Publication Reference
-
High-throughput identification of Toxoplasma gondii effector proteins that target host cell transcription.
Simon Butterworth, Kristina Kordova, Sambamurthy Chandrasekaran, Kaitlin K Thomas, Francesca Torelli, Eloise J Lockyer, Amelia Edwards, Robert Goldstone, Anita A Koshy, Moritz Treeck.
Cell Host & Microbe 2023 Oct; 31(10):1748.
Application：IF, Parasite, toxoplasma gondii.
-
Toxoplasma gondii virulence factor ROP1 reduces parasite susceptibility to murine and human innate immune restriction.
Simon Butterworth, Francesca Torelli, Eloise J Lockyer, Jeanette Wagener, Ok-Ryul Song, Malgorzata Broncel, Matt R G Russell, Aline Cristina A Moreira-Souza, Joanna C Young, Moritz Treeck.
PLoS Pathogens 2022 Dec; 18(12):e1011021.
Application：Co-IP, IF, WB-Ti, Human, Mouse, Human foreskin fibroblasts, Mouse embryonic fibroblasts.
-
High-throughput identification of Toxoplasma gondii effector proteins that target host cell transcription.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com