MIB1 monoclonal antibody, clone 6A9C9
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Specifications
Product Description
Mouse monoclonal antibody raised against recombinant human MIB1.
Immunogen
Recombinant protein corresponding to amino acid 6-221 of human MIB1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
110kDa
Reactivity
Human, Monkey
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell; Lane 2: COS-7 cell with MIB1 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) MIB1-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of HeLa cells with MIB1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of MIB1 monoclonal antibody, clone 6A9C9.Flow Cytometry
Flow cytometric analysis of HeLa cells with MIB1 monoclonal antibody (green) and negative control (red). -
Gene Info — MIB1
Entrez GeneID
57534Gene Name
MIB1
Gene Alias
DIP-1, DKFZp686I0769, DKFZp761M1710, FLJ90676, MGC129659, MGC129660, MIB, ZZANK2, ZZZ6
Gene Description
mindbomb homolog 1 (Drosophila)
Omim ID
608677Gene Ontology
HyperlinkOther Designations
DAPK-interacting protein 1|mindbomb homolog 1|ubiquitin ligase mind bomb
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Interactomes
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