MET monoclonal antibody, clone 5E9B7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) MET-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with MET monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of MET monoclonal antibody, clone 5E9B7.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells with MET monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human MET.
Immunogen
Recombinant protein corresponding to amino acid 743-932 of human MET from E. coli.
Host
Mouse
Theoretical MW (kDa)
155kDa
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:100-1:500)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) MET-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of HeLa cells with MET monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of MET monoclonal antibody, clone 5E9B7.Flow Cytometry
Flow cytometric analysis of MCF-7 cells with MET monoclonal antibody (green) and negative control (red). -
Gene Info — MET
Entrez GeneID
4233Gene Name
MET
Gene Alias
AUTS9, HGFR, RCCP2, c-Met
Gene Description
met proto-oncogene (hepatocyte growth factor receptor)
Gene Ontology
HyperlinkGene Summary
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
HGF receptor|OTTHUMP00000069168|SF receptor|met proto-oncogene|met proto-oncogene tyrosine kinase|oncogene MET|scatter factor receptor
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Interactome
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Pathway
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Disease
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