MET recombinant monoclonal antibody, clone 2D12
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: 293T whole cell lysate, Lane 2: Hela whole cell lysate, Lane 3: L02 whole cell lysate, Lane 4: PC3 whole cell lysate and Lane 5: A549 whole cell lysate with MET recombinant monoclonal antibody, clone 2D12 (Cat # RAB04393).
Immunohistochemistry
Immunohistochemical staining of human colon cancer with MET recombinant monoclonal antibody, clone 2D12 (Cat # RAB04393) (diluated at 1:100).
Immunohistochemistry
Immunohistochemical staining of human liver cancer with MET recombinant monoclonal antibody, clone 2D12 (Cat # RAB04393) (diluated at 1:100).
Immunofluorescence
Immunofluorescent staining of Hela cells with MET recombinant monoclonal antibody, clone 2D12 (Cat # RAB04393) (diluated at 1:50). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Flow Cytometry
Flow cytometric analysis of HeLa cells with MET recombinant monoclonal antibody, clone 2D12 (Cat # RAB04393) (diluated at 1:50; purple line) and negative control (green line).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human MET.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human MET.
Theoretical MW (kDa)
Calculated MW: 156,
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry (1:20-1:200)
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: 293T whole cell lysate, Lane 2: Hela whole cell lysate, Lane 3: L02 whole cell lysate, Lane 4: PC3 whole cell lysate and Lane 5: A549 whole cell lysate with MET recombinant monoclonal antibody, clone 2D12 (Cat # RAB04393).Immunohistochemistry
Immunohistochemical staining of human colon cancer with MET recombinant monoclonal antibody, clone 2D12 (Cat # RAB04393) (diluated at 1:100).Immunohistochemistry
Immunohistochemical staining of human liver cancer with MET recombinant monoclonal antibody, clone 2D12 (Cat # RAB04393) (diluated at 1:100).Immunofluorescence
Immunofluorescent staining of Hela cells with MET recombinant monoclonal antibody, clone 2D12 (Cat # RAB04393) (diluated at 1:50). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells with MET recombinant monoclonal antibody, clone 2D12 (Cat # RAB04393) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — MET
Entrez GeneID
4233Protein Accession#
P08581Gene Name
MET
Gene Alias
AUTS9, HGFR, RCCP2, c-Met
Gene Description
met proto-oncogene (hepatocyte growth factor receptor)
Gene Ontology
HyperlinkGene Summary
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
HGF receptor|OTTHUMP00000069168|SF receptor|met proto-oncogene|met proto-oncogene tyrosine kinase|oncogene MET|scatter factor receptor
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Interactome
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Pathway
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Disease
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