DNTT monoclonal antibody, clone 4B10A6
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Raji cell; Lane 2: A549 cell; Lane 3: HeLa cell; Lane 4: PC-12 cell with DNTT monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) DNTT-hIgGFc transfected HEK293 cell lysate.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DNTT monoclonal antibody, clone 4B10A6.
Flow Cytometry
Flow cytometric analysis of HeLa cells with DNTT monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human DNTT.
Immunogen
Recombinant protein corresponding to amino acid 52-192 of human DNTT from E. coli.
Host
Mouse
Theoretical MW (kDa)
58.5kDa
Reactivity
Human, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Raji cell; Lane 2: A549 cell; Lane 3: HeLa cell; Lane 4: PC-12 cell with DNTT monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) DNTT-hIgGFc transfected HEK293 cell lysate.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DNTT monoclonal antibody, clone 4B10A6.Flow Cytometry
Flow cytometric analysis of HeLa cells with DNTT monoclonal antibody (green) and negative control (red). -
Gene Info — DNTT
Entrez GeneID
1791Gene Name
DNTT
Gene Alias
TDT
Gene Description
deoxynucleotidyltransferase, terminal
Omim ID
187410Gene Ontology
HyperlinkGene Summary
This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq
Other Designations
DNA nucleotidylexotransferase|OTTHUMP00000020171|nucleosidetriphosphate:DNA deoxynucleotidylexotransferase|terminal addition enzyme|terminal deoxynucleotidyltransferase|terminal deoxyribonucleotidyltransferase|terminal transferase
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Interactome
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Pathway
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Disease
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