DNTT monoclonal antibody, clone RM379
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human DNTT.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human DNTT.
Reactivity
Human
Specificity
This antibody reacts to human DNTT.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:100-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Jurkat cell lysates with DNTT monoclonal antibody, clone RM379 (Cat # MAB23162) at a 1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human thymus with DNTT monoclonal antibody, clone RM379 (Cat # MAB23162) at a 1:200 dilution. -
Gene Info — DNTT
Entrez GeneID
1791Gene Name
DNTT
Gene Alias
TDT
Gene Description
deoxynucleotidyltransferase, terminal
Omim ID
187410Gene Ontology
HyperlinkGene Summary
This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq
Other Designations
DNA nucleotidylexotransferase|OTTHUMP00000020171|nucleosidetriphosphate:DNA deoxynucleotidylexotransferase|terminal addition enzyme|terminal deoxynucleotidyltransferase|terminal deoxyribonucleotidyltransferase|terminal transferase
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