BMP4 monoclonal antibody, clone 3C11C7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A549 cell; Lane 2: HepG2 cell; Lane 3: C6 cell with BMP4 monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) BMP4-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with BMP4 monoclonal antibody (green). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of BMP4 monoclonal antibody, clone 3C11C7.
Flow Cytometry
Flow cytometric analysis of HeLa cells with BMP4 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human BMP4.
Immunogen
Recombinant protein corresponding to amino acid 277-408 of human BMP4 from E. coli.
Host
Mouse
Theoretical MW (kDa)
46.5kDa
Reactivity
Human, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:100-1:500)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A549 cell; Lane 2: HepG2 cell; Lane 3: C6 cell with BMP4 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) BMP4-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of HeLa cells with BMP4 monoclonal antibody (green). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of BMP4 monoclonal antibody, clone 3C11C7.Flow Cytometry
Flow cytometric analysis of HeLa cells with BMP4 monoclonal antibody (green) and negative control (red). -
Gene Info — BMP4
Entrez GeneID
652Gene Name
BMP4
Gene Alias
BMP2B, BMP2B1, MCOPS6, ZYME
Gene Description
bone morphogenetic protein 4
Omim ID
112262Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein. [provided by RefSeq
Other Designations
bone morphogenetic protein 2B
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Interactome
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Pathway
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Disease
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