BMP4 recombinant monoclonal antibody, clone 19B7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Hela whole cell lysate with BMP4 recombinant monoclonal antibody, clone 19B7 (Cat # RAB07570).
Immunofluorescence
Immunofluorescent staining of Hela Cells with BMP4 recombinant monoclonal antibody, clone 19B7 (Cat # RAB07570), counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 508-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human BMP4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human BMP4.
Theoretical MW (kDa)
Calculated MW: 47
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Hela whole cell lysate with BMP4 recombinant monoclonal antibody, clone 19B7 (Cat # RAB07570).Immunofluorescence
Immunofluorescent staining of Hela Cells with BMP4 recombinant monoclonal antibody, clone 19B7 (Cat # RAB07570), counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 508-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).Enzyme-linked Immunoabsorbent Assay
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Gene Info — BMP4
Entrez GeneID
652Protein Accession#
P12644Gene Name
BMP4
Gene Alias
BMP2B, BMP2B1, MCOPS6, ZYME
Gene Description
bone morphogenetic protein 4
Omim ID
112262Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein. [provided by RefSeq
Other Designations
bone morphogenetic protein 2B
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Interactome
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Pathway
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Disease
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