DNMT3B monoclonal antibody, clone 4D1A2E5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) DNMT3B-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with DNMT3B monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DNMT3B monoclonal antibody, clone 4D1A2E5.
Flow Cytometry
Flow cytometric analysis of K562 cells with DNMT3B monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human DNMT3B.
Immunogen
Recombinant protein corresponding to amino acid 1-150 of human DNMT3B from E. coli.
Host
Mouse
Theoretical MW (kDa)
95.8kDa
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) DNMT3B-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of HeLa cells with DNMT3B monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DNMT3B monoclonal antibody, clone 4D1A2E5.Flow Cytometry
Flow cytometric analysis of K562 cells with DNMT3B monoclonal antibody (green) and negative control (red). -
Gene Info — DNMT3B
Entrez GeneID
1789Gene Name
DNMT3B
Gene Alias
ICF, M.HsaIIIB
Gene Description
DNA (cytosine-5-)-methyltransferase 3 beta
Gene Ontology
HyperlinkGene Summary
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq
Other Designations
DNA MTase HsaIIIB|DNA cytosine-5 methyltransferase 3 beta|DNA methyltransferase HsaIIIB
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Interactome
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Pathway
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Disease
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