DNMT3B polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant DNMT3B.
Immunogen
Recombinant protein corresponding to amino acids of human DNMT3B.
Sequence
MEPSPEPPSLESMKGDTRHLNGEEDAGGREDSILVNGACSDQSSDSPPILEAIRTPEIRGRRSSSRLSKREVSSLLSYTQDLTGDGDGEDGDGSDTPVMPKLFRETRTRSESPAVRTRNNNSVSSRERHRPSPRSTRGRQGRNHVDES
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with DNMT3B polyclonal antibody (Cat # PAB20073) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with DNMT3B polyclonal antibody (Cat # PAB20073) shows strong nuclear positivity in cells of seminiferus ducts at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with DNMT3B polyclonal antibody (Cat # PAB20073) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli, vesicles. -
Gene Info — DNMT3B
Entrez GeneID
1789Protein Accession#
Q9UBC3Gene Name
DNMT3B
Gene Alias
ICF, M.HsaIIIB
Gene Description
DNA (cytosine-5-)-methyltransferase 3 beta
Gene Ontology
HyperlinkGene Summary
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq
Other Designations
DNA MTase HsaIIIB|DNA cytosine-5 methyltransferase 3 beta|DNA methyltransferase HsaIIIB
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Interactome
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Disease
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