HSF1 monoclonal antibody, clone 4D5F4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: HSF1-hIgGFc transfected HEK293 cell with HSF1 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded rectal cancer tissues with HSF1 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of A431 cells with HSF1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of HSF1 monoclonal antibody, clone 4D5F4.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human HSF1.
Immunogen
Recombinant protein corresponding to amino acid 256-359 of human HSF1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
57.3
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: HSF1-hIgGFc transfected HEK293 cell with HSF1 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded rectal cancer tissues with HSF1 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of A431 cells with HSF1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of HSF1 monoclonal antibody, clone 4D5F4. -
Gene Info — HSF1
Entrez GeneID
3297Gene Name
HSF1
Gene Alias
HSTF1
Gene Description
heat shock transcription factor 1
Omim ID
140580Gene Ontology
HyperlinkGene Summary
The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq
Other Designations
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