HSF1 (phospho S326) monoclonal antibody, clone BHI-8
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human HSF1.
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding S326 of human HSF1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.4-0.5 mg/mL BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of HeLa cell lysates treated with heat using HSF1 (phospho S326) monoclonal antibody, clone BHI-8.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — HSF1
Entrez GeneID
3297Protein Accession#
Q00613Gene Name
HSF1
Gene Alias
HSTF1
Gene Description
heat shock transcription factor 1
Omim ID
140580Gene Ontology
HyperlinkGene Summary
The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq
Other Designations
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