CDK5RAP2 monoclonal antibody, clone CL3392
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with CDK5RAP2 monoclonal antibody, clone CL3392 (Cat # MAB15800) shows centrosome-like immunoreactivity in tumor cells.
Immunofluorescence
Immunofluorescent staining of HeLa cells with CDK5RAP2 monoclonal antibody, clone CL3392 (Cat # MAB15800) (Green) shows specific the centrosome. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human CDK5RAP2.
Immunogen
Recombinant protein corresponding to human CDK5RAP2.
Epitope
This antibody binds to an epitope located within the peptide sequence YETSEKSFFS as determined by overlapping synthetic peptides.
Sequence
ELLEKLEKLFLNGKSVGVEMNTQNELMERIEEDNLTYQHLLPESPEPSASHALSDYETSEKSFFSRDQKQDNETEKTSVMV
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with CDK5RAP2 monoclonal antibody, clone CL3392 (Cat # MAB15800) shows centrosome-like immunoreactivity in tumor cells.Immunofluorescence
Immunofluorescent staining of HeLa cells with CDK5RAP2 monoclonal antibody, clone CL3392 (Cat # MAB15800) (Green) shows specific the centrosome. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). -
Gene Info — CDK5RAP2
Entrez GeneID
55755Protein Accession#
Q96SN8Gene Name
CDK5RAP2
Gene Alias
C48, Cep215, DKFZp686B1070, DKFZp686D1070, KIAA1633, MCPH3
Gene Description
CDK5 regulatory subunit associated protein 2
Gene Ontology
HyperlinkGene Summary
Neuronal CDC2-like kinase, which is involved in the regulation of neuronal differentiation, is composed of a catalytic subunit, CDK5, and an activating subunit, p25NCK5A. The protein encoded by this gene binds to p25NCK5A and therefore may be involved in neuronal differentiation. The encoded protein may also be a substrate of neuronal CDC2-like kinase. Multiple transcript variants exist for this gene, but the full-length nature of only two has been determined. [provided by RefSeq
Other Designations
CDK5 activator-binding protein C48|OTTHUMP00000022800|OTTHUMP00000064031|centrosomal protein 215 kDa
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Interactome
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Disease
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