VPS26A monoclonal antibody, clone CL2287
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of U-251 MG cell lysate with VPS26A monoclonal antibody, clone CL2287 (Cat # MAB15737).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human fallopian tube with VPS26A monoclonal antibody, clone CL2287 (Cat # MAB15737) shows strong granular cytoplasmic immunoreactivity in glandular epithelium cells.
Immunofluorescence
Immunofluorescent staining of MCF7 cells with VPS26A monoclonal antibody, clone CL2287 (Cat # MAB15737) (Green) shows distinct endosomes. Microtubule and nuclear probes are visualized in red and blue respectively (where available).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human VPS26A.
Immunogen
Recombinant protein corresponding to human VPS26A.
Sequence
LRKQRTNFHQRFESPESQASAEQPEM
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:200-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of U-251 MG cell lysate with VPS26A monoclonal antibody, clone CL2287 (Cat # MAB15737).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human fallopian tube with VPS26A monoclonal antibody, clone CL2287 (Cat # MAB15737) shows strong granular cytoplasmic immunoreactivity in glandular epithelium cells.Immunofluorescence
Immunofluorescent staining of MCF7 cells with VPS26A monoclonal antibody, clone CL2287 (Cat # MAB15737) (Green) shows distinct endosomes. Microtubule and nuclear probes are visualized in red and blue respectively (where available). -
Gene Info — VPS26A
Entrez GeneID
9559Protein Accession#
O75436Gene Name
VPS26A
Gene Alias
FLJ12930, HB58, Hbeta58, PEP8A, VPS26
Gene Description
vacuolar protein sorting 26 homolog A (S. pombe)
Omim ID
605506Gene Ontology
HyperlinkGene Summary
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000019721|vacuolar protein sorting 26 A
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Interactome
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Disease
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