Golgi Complex monoclonal antibody, clone SPM581
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Specification
Product Description
Mouse monoclonal antibody raised against native human Golgi Complex.
Immunogen
SU-DHL-1 large cell lymphoma cells.
Host
Mouse
Reactivity
Human
Specificity
This antibody recognizes Golgi complex in human cells. It reacts specifically with human cells.
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (0.5-1.0 ug/million cells)
Immunocytochemistry (Acetone or paraformaldehyde fixed) (1-2 ug/mL for 30 min)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Western Blotting (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS.
Storage Instruction
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human gallbladder with Golgi Complex monoclonal antibody, clone SPM581 (Cat # MAB15231).Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Publication Reference
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Cellular localization and functional characterization of the equilibrative nucleoside transporters of antitumor nucleosides.
Endo Y, Obata T, Murata D, Ito M, Sakamoto K, Fukushima M, Yamasaki Y, Yamada Y, Natsume N, Sasaki T.
Cancer Science 2007 Oct; 98(10):1633.
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Binding and phosphorylation of a novel male germ cell-specific cGMP-dependent protein kinase-anchoring protein by cGMP-dependent protein kinase Ialpha.
Yuasa K, Omori K, Yanaka N.
The Journal of Biological Chemistry 2000 Feb; 275(7):4897.
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Cellular localization and functional characterization of the equilibrative nucleoside transporters of antitumor nucleosides.
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