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Last updated: 2017/12/10

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Mouse IgG2c monoclonal antibody, clone RM223

  • Catalog # : MAB15161
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against native mouse IgG2c.
  • Immunogen:
  • Native purified mouse IgG2c.
  • Host:
  • Rabbit
  • Reactivity:
  • Mouse
  • Specificity:
  • Recognizes the Fc region of mouse IgG2c. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.01-0.2 ug/mL)
    Flow Cytometry
    Immunocytochemistry (0.5-2 ug/mL)
    Immunohistochemistry (0.5-2 ug/mL)
    Immunoprecipitation
    Western Blotting (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western Blot analysis of nonreduced(-) and reduced(+) mouse IgG2c with Mouse IgG2c monoclonal antibody, clone RM223 (Cat # MAB15161).
  • Immunohistochemistry
  • Immunocytochemistry
  • ELISA
  • ELISA
  • ELISA analysis of Mouse IgG2c monoclonal antibody, clone RM223 (Cat # MAB15161) at the following concentrations: 200 ng/mL, 50 ng/mL and 10 ng/mL. The result shows this antibody reacts to the C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
  • Application Image
  • Immunohistochemistry
  • Immunocytochemistry
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