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ACACA monoclonal antibody, clone RM232

  • Catalog # : MAB15123
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human ACACA.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human ACACA.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:300-1:500)
    Western Blot (1:1000-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of A431 cell lysates with ACACA monoclonal antibody, clone RM232 (Cat # MAB15123) at a 1: 1,000 dilution, showed a band of ACACA (~260 kDa) expressed in A431 cells.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human heart with ACACA monoclonal antibody, clone RM232 (Cat # MAB15123) at 1:300 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 31
  • Gene Name:
  • ACACA
  • Gene Alias:
  • ACAC,ACC,ACC1,ACCA
  • Gene Description:
  • acetyl-Coenzyme A carboxylase alpha
  • Gene Summary:
  • Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • ACC-alpha,OTTHUMP00000164069,acetyl-CoA carboxylase 1,acetyl-CoA carboxylase-alpha
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