ACACA polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells, treated with PMA (125 ng/ml, 30 mins), using ACACA polyclonal antibody (Cat # PAB17282).
Peptide "+" means "with peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using ACACA polyclonal antibody (Cat # PAB17282).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ACACA.
Immunogen
A synthetic peptide corresponding to residues surrounding serine 79 of human ACACA.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total ACACA protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells, treated with PMA (125 ng/ml, 30 mins), using ACACA polyclonal antibody (Cat # PAB17282).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using ACACA polyclonal antibody (Cat # PAB17282).
Peptide "+" means "with peptide blocking". -
Gene Info — ACACA
Entrez GeneID
31Protein Accession#
Q13085Gene Name
ACACA
Gene Alias
ACAC, ACC, ACC1, ACCA
Gene Description
acetyl-Coenzyme A carboxylase alpha
Omim ID
200350Gene Ontology
HyperlinkGene Summary
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
ACC-alpha|OTTHUMP00000164069|acetyl-CoA carboxylase 1|acetyl-CoA carboxylase-alpha
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Interactome
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Pathway
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Disease
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Publication Reference
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Hepatic de novo lipogenesis is present in liver-specific ACC1-deficient mice.
Harada N, Oda Z, Hara Y, Fujinami K, Okawa M, Ohbuchi K, Yonemoto M, Ikeda Y, Ohwaki K, Aragane K, Tamai Y, Kusunoki J.
Molecular and Cellular Biology 2007 Jan; 27(5):1881.
Application：WB, Mouse, Mouse liver.
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Wheat cytosolic acetyl-CoA carboxylase complements an ACC1 null mutation in yeast.
Joachimiak M, Tevzadze G, Podkowinski J, Haselkorn R, Gornicki P.
PNAS 1997 Sep; 94(18):9990.
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Expression of the Acc1 Gene-Encoded Acetyl-Coenzyme A Carboxylase in Developing Maize (Zea mays L.) Kernels.
Somers DA, Keith RA, Egli MA, Marshall LC, Gengenbach BG, Gronwald JW, Wyse DL.
Plant Physiology 1993 Mar; 101(3):1097.
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Hepatic de novo lipogenesis is present in liver-specific ACC1-deficient mice.
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