KRT10 monoclonal antibody, clone SPM623
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Specifications
Product Description
Mouse monoclonal antibody raised against native human KRT10.
Immunogen
Cytoskeletal preparation extracted from human ectocervical epithelium.
Host
Mouse
Theoretical MW (kDa)
56.5
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
Immunofluorescence (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.1-0.2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS (0.05% BSA, 0.05% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human bladder carcinoma with KRT10 monoclonal antibody, clone SPM623 (Cat # MAB14576).Immunofluorescence
Flow Cytometry
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Gene Info — KRT10
Entrez GeneID
3858Protein Accession#
P13645Gene Name
KRT10
Gene Alias
CK10, K10, KPP
Gene Description
keratin 10
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq
Other Designations
cytokeratin 10
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Interactomes
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Publication Reference
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Absence of differentiation-related expression of keratin 10 in early stages of vulvar squamous carcinoma.
Ivanyi D, Ansink A, Mooi WJ, de Kraker NW, Heintz AP.
Differentiation 1989 Dec; 42(2):124.
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New monoclonal antibodies recognizing epidermal differentiation-associated keratins in formalin-fixed, paraffin-embedded tissue. Keratin 10 expression in carcinoma of the vulva.
Ivanyi D, Ansink A, Groeneveld E, Hageman PC, Mooi WJ, Heintz AP.
The Journal of Pathology 1989 Sep; 159(1):7.
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Absence of differentiation-related expression of keratin 10 in early stages of vulvar squamous carcinoma.
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