KRT10 recombinant monoclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot
Western blot analysis of rat skin (A), mouse skin (B), CHOK1 (C) whole cell lysates with Cytokeratin 10 recombinant monoclonal antibody (Cat # RAB02447).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human tonsil formalin fixed paraffin embedded tissue section using Cytokeratin 10 recombinant monoclonal antibody (Cat # RAB02447). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.115). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
-
Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human KRT10.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human Cytokeratin 10.
Theoretical MW (kDa)
58
Reactivity
Hamster, Human, Mouse, Rat
Specificity
Recognizes endogenous levels of Cytokeratin 10 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot
Western blot analysis of rat skin (A), mouse skin (B), CHOK1 (C) whole cell lysates with Cytokeratin 10 recombinant monoclonal antibody (Cat # RAB02447).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human tonsil formalin fixed paraffin embedded tissue section using Cytokeratin 10 recombinant monoclonal antibody (Cat # RAB02447). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.115). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Gene Info — KRT10
Entrez GeneID
3858Protein Accession#
P13645Gene Name
KRT10
Gene Alias
CK10, K10, KPP
Gene Description
keratin 10
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq
Other Designations
cytokeratin 10
-
Interactome
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com