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VIM monoclonal antibody, clone LN-6

  • Catalog # : MAB13452
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against native human VIM.
  • Immunogen:
  • Human thymic nuclear extract.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 57-60
  • Reactivity:
  • Human
  • Specificity:
  • This monoclonal antibody reacts with a non-hematopoietic epitope of VIM and shows no cross-reaction with other closely related intermediate filament proteins such as desmin, keratin, neurofilament, and glial fibrillary acid protein.
  • Form:
  • Liquid
  • Purification:
  • PEG precipitation
  • Isotype:
  • IgM
  • Recommend Usage:
  • Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
    Immunofluorescence (0.5-1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM PBS.
  • Storage Instruction:
  • Store at -20 to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human melanoma with VIM monoclonal antibody, clone LN-6 (Cat # MAB13452).
  • Immunofluorescence
  • Flow Cytometry
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunofluorescence
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 7431
  • Gene Name:
  • VIM
  • Gene Alias:
  • FLJ36605
  • Gene Description:
  • vimentin
  • Gene Summary:
  • This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract
  • Other Designations:
  • OTTHUMP00000019224
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