Product Browser

Last updated: 2017/2/19

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

Histone H2AX monoclonal antibody, clone RM214

  • Catalog # : MAB12802
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human Histone H2AX.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus region of Histone H2AX.
  • Host:
  • Rabbit
  • Specificity:
  • This antibody reacts to Histone H2AX protein, independent of post-translational modifications. No cross reactivity with other histone proteins. .
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.2 ug/mL-1 ug/mL)
    Immunocytochemistry (1 ug/mL-2 ug/mL)
    Western Blot (0.5 ug/mL-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: A375, Lane 2: HEK293, Lane 3: HeLa and Lane 4: SK-MEL-2 whole cell lysate with Histone H2AX monoclonal antibody, clone RM214 (Cat # MAB12802) at 0.5 ug/mL working concentration, showed endogenous Histone H2AX in A375, HEK293, HeLa and SK-MEL-2 cells.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemical staining of HeLa cells with Histone H2AX monoclonal antibody, clone RM214 (Cat # MAB12802) (Red). Actin filaments have been labeled with fluorescein phalloidin (Green).
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3014
  • Gene Name:
  • H2AFX
  • Gene Alias:
  • H2A.X,H2A/X,H2AX
  • Gene Description:
  • H2A histone family, member X
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq
  • Other Designations:
  • H2AX histone
  • RSS
  • YouTube
  • Linkedin
  • Facebook