EIF2S1 monoclonal antibody, clone AT5E10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate with EIF2S1 monoclonal antibody, clone AT5E10 (Cat # MAB12654) at 1:1000 dilution followed by HRP-conjugated goat anti-mouse secondary antibody and visualized by ECL detection system.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human small intestine with EIF2S1 monoclonal antibody, clone AT5E10 (Cat # MAB12654) at 10 ug/mL working concentration.
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Specification
Product Description
Mouse monoclonal antibody raised against human EIF2S1.
Immunogen
Recombinant protein corresponding to human EIF2S1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate with EIF2S1 monoclonal antibody, clone AT5E10 (Cat # MAB12654) at 1:1000 dilution followed by HRP-conjugated goat anti-mouse secondary antibody and visualized by ECL detection system.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human small intestine with EIF2S1 monoclonal antibody, clone AT5E10 (Cat # MAB12654) at 10 ug/mL working concentration.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EIF2S1
Entrez GeneID
1965Gene Name
EIF2S1
Gene Alias
EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A
Gene Description
eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Omim ID
603907Gene Ontology
HyperlinkGene Summary
The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM
Other Designations
eIF-2-alpha|eukaryotic translation initiation factor 2, subunit 1 (alpha, 35kD )
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Interactome
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Disease
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