KRT18 monoclonal antibody, clone AT2G3
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Specification
Product Description
Mouse monoclonal antibody raised against human KRT18.
Immunogen
Recombinant protein corresponding to amino acids 79-430 of human KRT18.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Immunofluorescence (1:500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell lysate and Lane 2: AGS cell lysate with KRT18 monoclonal antibody, clone AT2G3 (Cat # MAB12652) at 1:1000 dilution followed by HRP-conjugated goat anti-mouse secondary antibody and visualized by ECL detection system.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skin with KRT18 monoclonal antibody, clone AT2G3 (Cat # MAB12652) at 10 ug/mL working concentration.Immunofluorescence
Immunofluorescent staining of HeLa cells with KRT18 monoclonal antibody, clone AT2G3 (Cat # MAB12652) at 1:500 dilution.Enzyme-linked Immunoabsorbent Assay
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Gene Info — KRT18
Entrez GeneID
3875Gene Name
KRT18
Gene Alias
CYK18, K18
Gene Description
keratin 18
Gene Ontology
HyperlinkGene Summary
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
cell proliferation-inducing protein 46|cytokeratin 18
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Interactome
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Pathway
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Disease
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