CAPN1 monoclonal antibody, clone 1376CT809.24.85
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A431 cell line lysates Lane 2: A549 cell line lysates Lane 3: MCF-7 cell line lysates Lane 4: U-251 MG cell line lysates reacted with CAPN1 monoclonal antibody (Cat # MAB12351) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human kidney section reacted with CAPN1 monoclonal antibody (Cat # MAB12351) at 1:25 dilution.
Flow Cytometry
Flow cytometric analysis of Hela cells (green histogram) and an isotype control of mouse IgG1 (blue histogram) reacted with CAPN1 monoclonal antibody (Cat # MAB12351) at 1:25 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human CAPN1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to human CAPN1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (1:25)
Immunohistochemistry (1:25)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A431 cell line lysates Lane 2: A549 cell line lysates Lane 3: MCF-7 cell line lysates Lane 4: U-251 MG cell line lysates reacted with CAPN1 monoclonal antibody (Cat # MAB12351) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human kidney section reacted with CAPN1 monoclonal antibody (Cat # MAB12351) at 1:25 dilution.Flow Cytometry
Flow cytometric analysis of Hela cells (green histogram) and an isotype control of mouse IgG1 (blue histogram) reacted with CAPN1 monoclonal antibody (Cat # MAB12351) at 1:25 dilution. -
Gene Info — CAPN1
Entrez GeneID
823Gene Name
CAPN1
Gene Alias
CANP, CANP1, CANPL1, muCANP, muCL
Gene Description
calpain 1, (mu/I) large subunit
Omim ID
114220Gene Ontology
HyperlinkGene Summary
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. [provided by RefSeq
Other Designations
calcium-activated neutral proteinase|calpain 1, large subunit|calpain, large polypeptide L1|cell proliferation-inducing protein 30
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Interactome
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Pathway
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