CAPN1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CAPN1.
Immunogen
A synthetic peptide corresponding to amino acids 468-481 at internal region of human CAPN1.
Sequence
NASRARSEQFINLR
Host
Goat
Theoretical MW (kDa)
80
Reactivity
Chicken, Human, Pig
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Western Blot (0.3-1ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CAPN1 polycolnal antibody (Cat # PAB27675) (0.3ug/ml) staining of human kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. -
Gene Info — CAPN1
Entrez GeneID
823Protein Accession#
NP_005177.2Gene Name
CAPN1
Gene Alias
CANP, CANP1, CANPL1, muCANP, muCL
Gene Description
calpain 1, (mu/I) large subunit
Omim ID
114220Gene Ontology
HyperlinkGene Summary
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. [provided by RefSeq
Other Designations
calcium-activated neutral proteinase|calpain 1, large subunit|calpain, large polypeptide L1|cell proliferation-inducing protein 30
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