PPT1 monoclonal antibody, clone 1117CT11.2.1.4
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human PPT1.
Immunogen
Recombinant protein corresponding to full length human PPT1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
Flow Cytometry (1:25)
Immunohistochemistry (1:25)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela whole cell lysates Lane 2: HepG2 whole cell lysates reacted with PPT1 monoclonal antibody (Cat # MAB12348) at 1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human cerebellum section reacted with PPT1 monoclonal antibody (Cat # MAB12348) at 1:25 dilution.Flow Cytometry
Flow cytometric analysis of HepG2 cells (green histogram) and an isotype control of mouse IgG1 (blue histogram) reacted with PPT1 monoclonal antibody (Cat # MAB12348) at 1:25 dilution. -
Gene Info — PPT1
Entrez GeneID
5538Gene Name
PPT1
Gene Alias
CLN1, INCL, PPT
Gene Description
palmitoyl-protein thioesterase 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene
Other Designations
OTTHUMP00000004836|ceroid-palmitoyl-palmitoyl-protein thioesterase 1|palmitoyl-protein hydrolase 1
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Interactome
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