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PPT1 monoclonal antibody, clone 1117CT11.2.1.4

  • Catalog # : MAB12348
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant human PPT1.
  • Immunogen:
  • Recombinant protein corresponding to full length human PPT1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Flow Cytometry (1:25)
    Immunohistochemistry (1:25)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: Hela whole cell lysates Lane 2: HepG2 whole cell lysates reacted with PPT1 monoclonal antibody (Cat # MAB12348) at 1:2000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of paraffin-embedded human cerebellum section reacted with PPT1 monoclonal antibody (Cat # MAB12348) at 1:25 dilution.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HepG2 cells (green histogram) and an isotype control of mouse IgG1 (blue histogram) reacted with PPT1 monoclonal antibody (Cat # MAB12348) at 1:25 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5538
  • Gene Name:
  • PPT1
  • Gene Alias:
  • CLN1,INCL,PPT
  • Gene Description:
  • palmitoyl-protein thioesterase 1
  • Gene Summary:
  • The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene
  • Other Designations:
  • OTTHUMP00000004836,ceroid-palmitoyl-palmitoyl-protein thioesterase 1,palmitoyl-protein hydrolase 1
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