Flow Cytometry (0.5-1 ug/million cells) Immunofluorescence (1-2 ug/mL) Immunohistochemistry (Frozen sections) (1-2 ug/mL for 30 min at RT) Immunoprecipitation (1-2 ug/500 ug protein) Western Blot (0.5-1 ug/mL) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.05% BSA, 0.05% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq