TNFSF13B monoclonal antibody, clone H4-C7
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of rat spleen tissue lysate.
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with TNFSF13B monoclonal antibody, clone H4-C7 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometry analysis of PBMCs cell line, staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant TNFSF13B.
Immunogen
Recombinant protein corresponding to amino acids 134-285 of human TNFSF13B.
Host
Mouse
Reactivity
Human, Rat
Form
Liquid
Purification
Protein G purification
Isotype
IgG3, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of rat spleen tissue lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with TNFSF13B monoclonal antibody, clone H4-C7 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of PBMCs cell line, staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — TNFSF13B
Entrez GeneID
10673GeneBank Accession#
NM_006573Protein Accession#
NP_006564Gene Name
TNFSF13B
Gene Alias
BAFF, BLYS, CD257, DTL, TALL-1, TALL1, THANK, TNFSF20, ZTNF4
Gene Description
tumor necrosis factor (ligand) superfamily, member 13b
Omim ID
603969Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. [provided by RefSeq
Other Designations
ApoL related ligand TALL-1|B-cell activating factor|B-lymphocyte stimulator|OTTHUMP00000018691|TNF and ApoL-related leukocyte expressed ligand 1|TNF homolog that activates apoptosis|delta BAFF|dendritic cell-derived TNF-like molecule|tumor necrosis factor
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Interactome
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Pathway
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Disease
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Publication Reference
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Genetic polymorphisms of interleukin-1B (IL-1B), IL-6, IL-8, and IL-10 and risk of prostate cancer.
Michaud DS, Daugherty SE, Berndt SI, Platz EA, Yeager M, Crawford ED, Hsing A, Huang WY, Hayes RB.
Cancer Research 2006 Apr; 66(8):4525.
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Interleukin-1 and neuronal injury.
Allan SM, Tyrrell PJ, Rothwell NJ.
Nature Reviews. Immunology 2005 Aug; 5(8):629.
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Genetic polymorphisms of interleukin-1B (IL-1B), IL-6, IL-8, and IL-10 and risk of prostate cancer.
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